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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Xiong, X., Xiong, F., Tian, H., Wang, Z., Wang, Y., Tao, R., Klausen, L. H. & Dong, M. (2022). Ultrathin Anion Conductors Based Memristor. Advanced Electronic Materials, 8(2), Article 2100845. https://doi.org/10.1002/aelm.202100845

Meisl, G., Xu, C. K., Taylor, J. D., Michaels, T. C. T., Levin, A., Otzen, D., Klenerman, D., Matthews, S., Linse, S., Andreasen, M. & Knowles, T. P. J. (2022). Uncovering the universality of self-replication in protein aggregation and its link to disease. Science Advances, 8(32), Article eabn6831. https://doi.org/10.1126/sciadv.abn6831

Klein, V. J., Irla, M., López, M. G., Brautaset, T. & Brito, L. F. (2022). Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy. Microorganisms, 10(2), Article 220. https://doi.org/10.3390/microorganisms10020220

Pothoulakis, G., Nguyen, M. T. A. & Andersen, E. S. (2022). Utilizing RNA origami scaffolds in Saccharomyces cerevisiae for dCas9-mediated transcriptional control. Nucleic Acids Research, 50(12), 7176-7187. https://doi.org/10.1093/nar/gkac470

Neumann, C., Focht, D., Trampari, S., Lyons, J. A. & Nissen, P. (2022). Variations on a theme: Crystal forms of the amino-acid transporter MhsT. Acta Crystallographica Section F:Structural Biology Communications, 78(Part 8), 297-305. https://doi.org/10.1107/S2053230X22007154

Nielsen, K. H. (2022). Vores allesammens virus. Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2022). Webb eller woke? Weekendavisen, Sektion 4 (Ideer), 5.

Zhang, Y., Le Friec, A. & Chen, M. (2021). 3D anisotropic conductive fibers electrically stimulated myogenesis. International Journal of Pharmaceutics, 606, 120841. Article 120841. https://doi.org/10.1016/j.ijpharm.2021.120841

Su, Y., Toftdal, M. S., Friec, A. L., Dong, MD., Han, X. & Chen, M. (2021). 3D Electrospun Synthetic Extracellular Matrix for Tissue Regeneration. Small Science, 1(7), Article 2100003. https://doi.org/10.1002/smsc.202100003

Su, Y., Li, Q., Amagat, J. & Chen, M. (2021). 3D spring-based piezoelectric energy generator. Nano Energy, 90, Article 106578. https://doi.org/10.1016/j.nanoen.2021.106578

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