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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Søgaard, A. B., Hansson, R. F., Tvilum, A. S. & Zelikin, A. N. (2024). Artificial Internalizing Receptors: Intracellular Delivery of Cargo Through Bio-Orthogonal Recognition. Advanced Healthcare Materials, 13(32), Article 2402472. https://doi.org/10.1002/adhm.202402472

Basse-O’connor, A. & Podolskij, M. (2024). Asymptotic theory for quadratic variation of harmonizable fractional stable processes. Theory of Probability and Mathematical Statistics, 110, 3-12. https://doi.org/10.1090/tpms/1203

Zhang, J., Traspas Muiña, A., Mifsud, D. V., Kaňuchová, Z., Cielinska, K., Herczku, P., Rahul, K. K., Kovács, S. T. S., Rácz, R., Santos, J. C., Hopkinson, A. T., Craciunescu, L., Jones, N. C., Hoffmann, S. V., Biri, S., Vajda, I., Rajta, I., Dawes, A., Sivaraman, B. ... Ioppolo, S. (2024). A systematic IR and VUV spectroscopic investigation of ion, electron, and thermally processed ethanolamine ice. Monthly Notices of the Royal Astronomical Society, 533(1), 826-840. https://doi.org/10.1093/mnras/stae1860

Santos, J., Cuellar, J., Pallarès, I., Byrd, E. J., Lends, A., Moro, F., Abdul-Shukkoor, M. B., Pujols, J., Velasco-Carneros, L., Sobott, F., Otzen, D. E., Calabrese, A. N., Muga, A., Pedersen, J. S., Loquet, A., Valpuesta, J. M., Radford, S. E. & Ventura, S. (2024). A Targetable N-Terminal Motif Orchestrates α-Synuclein Oligomer-to-Fibril Conversion. Journal of the American Chemical Society, 146(18), 12702-12711. https://doi.org/10.1021/jacs.4c02262

Vieira de Sá, R., Sudria-Lopez, E., Cañizares Luna, M., Harschnitz, O., van den Heuvel, D. M. A., Kling, S., Vonk, D., Westeneng, H. J., Karst, H., Bloemenkamp, L., Varderidou-Minasian, S., Schlegel, D. K., Mars, M., Broekhoven, M. H., van Kronenburg, N. C. H., Adolfs, Y., Vangoor, V. R., de Jongh, R., Ljubikj, T. ... Pasterkamp, R. J. (2024). ATAXIN-2 intermediate-length polyglutamine expansions elicit ALS-associated metabolic and immune phenotypes. Nature Communications, 15(1), Article 7484. https://doi.org/10.1038/s41467-024-51676-0

Otzen, D. & d'Angelo, J. (2024). BBA: 2024 and beyond. Biochimica et Biophysica Acta - General Subjects, 1868(2), Article 130524. https://doi.org/10.1016/j.bbagen.2023.130524

Nielsen, K. H. (2024). Beskedne genier. Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2024). Bestilt, bekvem våbenforskning. Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2024). Blitzkrig i haven. Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2024). Blomstens følelser. Weekendavisen, Sektion 4 (Ideer), 5.

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