Publications

Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

Sort by: Date | Author | Title

Nielsen, K. H. (2006). Anmeldelse af: David Finer, Fokus Forskning (2005).

Nielsen, K. H. (2006). Anmeldelse af: Jesper Gulddal, red., Pas: identitet, kultur og grænser (2004). Nord Nytt, (98), 119.

Nielsen, K. H. (2006). Anmeldelse af: Ludwig Wittgenstein, Den ukjente dagboken (2004). Nord Nytt, (98), 109.

Lützelberger, M., Reinert, L. S., Das, A. T., Berkhout, B. & Kjems, J. (2006). A novel splice donor site in the gag-pol gene is required for HIV-1 RNA stability. Journal of Biological Chemistry, 281(27), 18644-18651. https://doi.org/10.1074/jbc.M513698200

Lomstein, B. A., Guldberg, L. B., Neubauer, A. T. A., Hansen, J., Donnelly, A., Herbert, R. A., Viaroli, P., Giordani, G., Azzoni, R., de Wit, R. & Finster, K. (2006). Benthic decomposition of Ulva lactuca: A controlled laboratory experiment. Aquatic Botany, 85(4), 271-281. https://doi.org/10.1016/j.aquabot.2006.05.006

Greunke, K., Spillner, E., Braren, I., Seismann, H., Kainz, S., Hahn, U., Grunwald, T. & Bredehorst, R. (2006). Bivalent monoclonal IgY antibody formats by conversion of recombinant antibody fragments. Journal of Biotechnology, 124(2), 446-56. https://doi.org/10.1016/j.jbiotec.2005.12.032

Ebbesen, M. (2006). Brug af nanoteknologi inden for fødevareforskning. In S. Brock, A. Moe Rasmussen & B. B. Hansen (Eds.), Tankeføde - om mad, måltider og fødevarer i sammenhæng (pp. 119-135). Forlaget Philosophia.

Hornekær, L., Rauls, E., Xu, W., Sljivancanin, Z., Otero, R., Stensgaard, I., Lægsgaard, E., Hammer, B. & Besenbacher, F. (2006). Clustering of Chemisorbed H(D) Atoms on the Graphite (0001) Surface due to Preferential Sticking. Physical Review Letters, 97, 186102.

Xu, S., Dong, M., Rauls, E., Otero Martin, R., Linderoth, T. R. & Besenbacher, F. (2006). Co-adsorption of guanine and cytosine on graphite: ordered structure based GC pairing. Nano Letters, 6(7), 1434-1438.

Sørensen, T. L.-M., Olesen, C., Jensen, A.-M. L., Møller, J. V. & Nissen, P. (2006). Crystals of sarcoplasmic reticulum Ca²⁺-ATPase. Journal of Biotechnology, 124(4), 704-716.

Displaying results 3141 to 3150 out of 3469

315