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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Ebbesen, M. & Pedersen, B. D. (2007). Using Empirical Ethics to Formulate Normative Ethical Principles in Biomedicine. Medicine, Health Care and Philosophy, (1), 33-48.

Hansen, A. A., Herbert, R., Mikkelsen, K., Jensen, L. L., Kristoffersen, T., Tiedje, J. M., Lomstein, B. A. & Finster, K. (2007). Viability, diversity and composition of the bacterial community in a high Arctic permafrost soil from Spitsbergen, Northern Norway. Environmental Microbiology, 9(11), 2870-2884.

Dong, M., Howard, K. A., Oupicky, D., Bisht, H. J., Kjems, J. & Besenbacher, F. (2007). Visualisation of Thermal Activated Nanocarriers Using In Situ Atomic Force Microscopy. Nanotechnology.

Dong, M. D., Howard, K. A., Oupicky, D., Bisht, H., Kjems, J. & Besenbacher, F. (2007). Visualization of thermally activated nanocarriers using in situ atomic force microscopy: Nanotechnology. Nanotechnology, 18(18), Article 185501. https://doi.org/10.1088/0957-4484/18/18/185501

Otzen, D. & Nielsen, P. H. (2007). We find them here, we find them there: Functional bacterial amyloid. Cellular and Molecular Life Sciences, 65, 910-927.

Nielsen, K. H. (2006). 100 års videnskabsformidling. In R. S. Kjærgaard (Ed.), Elfenbenstårnet: Universiteter mellem forskning og formidling (pp. 35-54). Aarhus Universitetsforlag.

Dong, MD., Hovgaard, M. B., Xu, S., Otzen, D. E. & Besenbacher, F. (2006). AFM study of glucagon fibrillation via oligomeric structures resulting in interwoven fibrils. Nanotechnology, (17), 4003-4009.

Ebbesen, M. (2006). A Journey into the World of Atoms and Molecules. Journal of Lutheran Ethics , (2).

Jonstrup, S. P., Koch, J. & Kjems, J. (2006). A microRNA detection system based on padlock probes and rolling circle amplification. RNA, 12(9), 1747-1752. https://doi.org/10.1261/rna.110706

Nielsen, K. H. (2006). Anmeldelse af: Claus Holm: Forståelsens rasen (2005).

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