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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Dirac, A. M., Huthoff, H., Kjems, J. & Berkhout, B. (2001). The DIS hairpin mediates dimerization of the HIV-2 RNA genome. Journal of Biological Chemistry, 276, 32345-32352.

Tange, T. Ø., Damgaard, C. K., Guth, S., Valcárcel, J. & Kjems, J. (2001). The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element. EMBO Journal, 20(20), 5748-5758. https://doi.org/10.1093/emboj/20.20.5748

Merrison, J. P., Field, D., Finster, K., Lomstein, B. A., Nørnberg, P., Ramsing, N. B. & Uggerhøj, E. (2001). The Mars Simulation Laboratory, University of Aarhus. (pp. 371-374).

Sørensen, K. B., Finster, K. & Ramsing, N. B. (2001). Thermodynamic and Kinetic Requirements in Anaerobic Methane Oxidizing Consortia Exclude Hydrogen, Acetate, and Methanol as Possible Electron Shuttles. Microb. Ecol., 42, 1-10.

Kammler, S., Leurs, C., Krummheuer, J., Seidel, K., Freund, M., Tange, T., Kjems, J., Scheid, A. & Schaal, H. (2001). The sequence complementarity of the HIV-1 5′ splice site SD4 and of U1 snRNA determines the steady-state level of an instable env pre-mRNA. RNA, 7, 421-434.

Nielsen, K. H. (2001). Vindmøller laver ikke vindkraft. In Danmarks Tekniske Museum: Årbog 2000 (Vol. 46, pp. 44-59). Danmarks Tekniske Museum.

Lund, M., Dyhr-Mikkelsen, H., Hansen, J. & Kjems, J. (2000). Characterization of human RNA splice signals by iterative functional selection of splice sites. RNA, 6, 528-544.

Madsen, N., Bowe, P., Drewsen, M., Hornekær, L., Kjærgaard, N., Labrador, A., Nielsen, J. S., Schiffer, J. P., Shi, P. & Hangst, J. S. (2000). Density distribution in laser-cooled bunched beams. Nucl. Inst. & Meth, A 441, 203.

Ebbesen, M. (2000). Det syge og det sunde. Information.

Sørensen, T. L.-M., Dupont, Y., Vilsen, B. & Andersen, J. P. (2000). Fast kinetic analysis of conformational changes in mutants of the Ca²⁺-ATPase of sarcoplasmic reticulum. Journal of Biological Chemistry, 275, 5400-5408. https://doi.org/10.1074/jbc.275.8.5400

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