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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Olesen, J. R., Kjems, J. & Besenbacher, F. (2006). Småt blir' godt: Nanomedicin i fremtidens sundhedssektor. Lægen i Midten, (5), 31.

Lützelberger, M. & Kjems, J. (2006). Strategies to identify potential therapeutic target sites in RNA. In V. Erdmann, J. Barciszewski & J. Brosius (Eds.), Handbook of Experimental Pharmacology: RNA Towards Medicine (Vol. 173, pp. 243-259). Springer. https://doi.org/10.1007/b138836

Pedersen, J. S., Flink, J. M., Dikov, D. & Otzen, D. (2006). Sulfates Dramatically Stabilize a Salt-Dependent Type of Glucagon Fibrils. Biophysical Journal, 90(11), 4181-4194. https://doi.org/10.1529/biophysj.105.070912

Mamdouh, W., Dong, M., Xu, S., Rauls, E. & Besenbacher, F. (2006). Supramolecular nanopatterns self-assembled by adenine-thymine quartets at the liquid/solid interface. Journal of the American Chemical Society, 128(40), 13305-11. https://doi.org/10.1021/ja064988u

Mamdouh, W., Dong, M., Xu, S., Rauls, E. & Besenbacher, F. (2006). Supramolecular Nanopatterns Self-Assembled by Adenine-Thymine Quartets at the Liquid/Solid Interface. Journal of the American Chemical Society, 128, 13305-13311. https://doi.org/10.1021/ja064988u

Paca-Uccaralertkun, S., Damgaard, C. K., Auewarakul, P., Thitithanyanont, A., Suphaphiphat, P., Essex, M., Kjems, J. & Lee, T.-H. (2006). The Effect of a Single Nucleotide Substitution in the Splicing Silencer in the tat/rev Intron on HIV Type 1 Envelope Expression. AIDS Research and Human Retroviruses, 22(1), 76-82. https://doi.org/10.1089/aid.2006.22.76

Hornekær, L., Baurichter, A., Petrunin, V. V. & Luntz, A. C. (2006). The influence of dust grainmorphology on H₂ formation and desorption in the interstellar medium. Proceedings: The dusty and molecular universe, ESA SP-577, 369.

Sehgal, P. & Otzen, D. (2006). Thermodynamics of unfolding of an integral membrane protein in mixed micelles. Protein Science, 15, 890-899. https://doi.org/10.1110/ps.052031306

Kammler, S., Otte, M., Hauber, I., Kjems, J., Hauber, J. & Schaal, H. (2006). The strength of the HIV-1 3' splice sites affects Rev function. Retrovirology, 3(89), 1-20. https://doi.org/10.1186/1742-4690-3-89

Andersen, E. S. (2006). The Structure, Function and Evolution of HIV-1 RNA. Aarhus University.

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