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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Basse-O'Connor, A. & Rosinski, J. (2013). On the uniform convergence of random series in Skorohod space and representations of càdlàg infinitely divisible processes. Annals of Probability, 41(6), 4317-4341. https://doi.org/10.1214/12-AOP783

Li, J., Han, Q., Zhang, Y., Yang, R., Wang, C., Zhang, W., Dong, M. & Besenbacher, F. (2013). Optical regulation of protein adsorption and cell adhesion by photoresponsive GaN nanowires. A C S Applied Materials and Interfaces, 5(19), 9816-9822. https://doi.org/10.1021/am403070g

Antaloae, A. V., Montigny, C., le Maire, M., Watson, K. A. & Sørensen, T. L.-M. (2013). Optimisation of recombinant production of active human cardiac SERCA2a ATPase. PLoS One, 8(8), e71842. https://doi.org/10.1371/journal.pone.0071842

Iversen, F., Yang, C., Dagnæs-Hansen, F., Schaffert, D. H., Kjems, J. & Gao, S. (2013). Optimized siRNA-PEG Conjugates for Extended Blood Circulation and Reduced Urine Excretion in Mice. Theranostics, 3(3), 201-209. https://doi.org/10.7150/thno.5743

Nawroth, I., Alsner, J., Deleuran, B. W., Dagnæs-Hansen, F., Yang, C., Horsman, M. R., Overgaard, J., Howard, K., Kjems, J. & Gao, S. (2013). Peritoneal macrophages mediated delivery of chitosan/siRNA nanoparticle to the lesion site in a murine radiation-induced fibrosis model. Acta Oncologica, 52(8), 1730-1738. https://doi.org/10.3109/0284186X.2012.726373

Wang, H., Andersen, K. K., Sehgal, P., Hagedorn, J., Westh, P., Borch, K. & Otzen, D. E. (2013). pH regulation of the kinetic stability of the lipase from Thermomyces lanuginosus. Biochemistry, 52(1), 264-276. https://doi.org/10.1021/bi301258e

Blank, S., Neu, C., Hasche, D., Bantleon, F. I., Jakob, T. & Spillner, E. (2013). Polistes species venom is devoid of carbohydrate-based cross-reactivity and allows interference-free diagnostics. The Journal of Allergy and Clinical Immunology, 131(4), 1239-42. https://doi.org/10.1016/j.jaci.2012.10.047

Andreasen, S. Ø., Chong, S. F., Wohl, B. M., Goldie, K. N. & Zelikin, A. N. (2013). Poly(vinyl alcohol) Physical Hydrogel Nanoparticles, Not Polymer Solutions, Exert Inhibition of Nitric Oxide Synthesis in Cultured Macrophages. Biomacromolecules, 14, 1687-1695. https://doi.org/10.1021/bm400369u

Chu, L., Xue, Q., Sun, J., Xia, F., Xing, W., Xia, D. & Dong, M. (2013). Porous graphene sandwich/poly(vinylidene fluoride) composites with high dielectric properties. Composites Science and Technology, 86, 70-75. https://doi.org/10.1016/j.compscitech.2013.07.001

Lin, L., Zhang, H., Cui, H., Xu, M., Cao, S., Zheng, G. & Dong, M. (2013). Preparation and antibacterial activities of hollow silica-Ag spheres. Colloids and Surfaces B: Biointerfaces, 101, 97-100. https://doi.org/10.1016/j.colsurfb.2012.06.001

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Kalinka Hansen