Find

AUNAB - Aarhus University Network on Artificial Biology

Publications

Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

Sort by: Date | Author | Title

Pallesen, J., Dong, M., Besenbacher, F. & Kjems, J. (2009). Structure of the HIV-1 Rev response element alone and in complex with regulator of virion (Rev) studied by atomic force microscopy. FEBS journal, 276(15), 4223-32. https://doi.org/10.1111/j.1742-4658.2009.07130.x

Andersen, L. N. (2009). Subexponential loss rate asymptotics for Lévy processes. Thiele Centre, Institut for Matematiske Fag, Aarhus Universitet.

Cabré, E., Malmström, J., Sutherland, D., Perez-Gil, J. & Otzen, D. (2009). Surfactant protein SP-B strongly modifies surface collapse of phospholipid vesicles: Insights from a quartz crystal microbalance with dissipation. Biophysical Journal, 97(3), 768-76. https://doi.org/10.1016/j.bpj.2009.04.057

Mittag, T., Nielsen, N. C., Otzen, D. & Skrydstrup, T. (2009). Synthesis of a Ketomethylene Isostere of the Fibrillating Peptide SNNFGAILSS. Journal of Organic Chemistry, 74, 7955-7957.

Hjørringgaard, C. U., Vad, B. S., Nielsen, S. B., Nielsen, N. C., Otzen, D. & Skrydstrup, T. (2009). Templated Multimers of Antimicrobial Peptides. Poster session presented at 8th Australian Peptide Conference, Peptides - Tools, Targets & Therapeutics , Australia.

Yan, K., Xue, Q., Xia, D., Chen, H., Xie, J. & Dong, M. (2009). The core/shell composite nanowires produced by self-scrolling carbon nanotubes onto copper nanowires. A C S Nano, 3(8), 2235-40. https://doi.org/10.1021/nn9005818

Gao, S., Dagnæs-Hansen, F., Nielsen, E. J. B., Wengel, J., Besenbacher, F., Howard, K. A. & Kjems, J. (2009). The effect of chemical modification and nanoparticle formulation on stability and biodistribution of siRNA in mice. Molecular Therapy, 17(7), 1225-1233. https://doi.org/10.1038/mt.2009.91

Hobolth, A. & Wiuf, C. H. (2009). The genealogy, site frequency spectrum and ages of two nested mutant alleles. Theoretical Population Biology, 75(4), 260-265. https://doi.org/10.1016/j.tpb.2009.02.001

Kjær, L., Giehm, L., Heimburg, T. R. & Otzen, D. (2009). The influence of vesicle composition and size on a-synuclein structure and stability. Biophysical Journal, 96.

Ebbesen, M. (2009). The Principle of Justice and Access to Nanomedicine in National Healthcare Systems. Studies in Ethics, Law, and Technology, (3), 1-15.

Displaying results 2951 to 2960 out of 3469

296

Revised 03.03.2026

-

Kalinka Hansen