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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Nielsen, K. H. (2008). Visual framing of nanotechnology in newspapers. Abstract from PCST-10 Bridges to the future, Malmø, Sweden.

Otzen, D., Sehgal, P. & Nesgaard, L. (2007). Alternative membrane protein conformations in alcohol. Biochemistry, (46), 4348-4359.

Lykke-Andersen, S., Piñol-Roma, S. & Kjems, J. (2007). Alternative splicing of the ADAR1 transcript in a region that functions either as a 5'-UTR or an ORF. RNA, 13(10), 1732-1744. https://doi.org/10.1261/rna.567807

Larsen, P., Dueholm, M. S., Christiansen, G., Nielsen, J. L., Otzen, D. & Nielsen, P. H. (2007). Amyloid adhesins are abundant in natural biofilms. Env. Microbiol., (9), 3077-3090.

Finster, K. (2007). Anaerobic bacteria and archaea in cold ecosystems. In R. Margesin, F. Schinner, J.-C. Marx & C. Gerday (Eds.), Psychrophiles: From biodiversity to biotechnology (pp. 103-118). Springer LNCS.

Dupont, D. M., Madsen, J. B., Kjems, J. & Andreasen, P. (2007). A novel type of agent blocking the association of uPA to its receptor uPAR: uPA-binding aptamers. Abstract from XIth International Workshop on Molecular and Cellular Biology of Plasminogen Activation, Stockholm, Sweden.

Dauksaite, V., Lorentzenn, M., Besenbacher, F. & Kjems, J. (2007). Antibody-based protein detection using piezoresistive cantilever array. Nanotechnology, 18, 1-5.

Frandsen, J., Hobolth, A., Østergaard, L., Vestergaard-Poulsen, P. & Jensen, E. B. V. (2007). Bayesian regularization of diffusion tensor images. Biostatistics, 8(4), 784-799. https://doi.org/10.1093/biostatistics/kxm005

Picard, M., Jensen, A.-M. L., Sørensen, T. L.-M., Champeil, P., Møller, J. V. & Nissen, P. (2007). Ca(2+) versus Mg(2+) Coordination at the Nucleotide-binding site of the Sarcoplasmic Reticulum Ca(2+)-ATPase. Journal of Molecular Biology, 368(1), 1-7.

Dalsgaard, T. K., Otzen, D., Nielsen, J. H. & Larsen, L. B. (2007). Changes in structures of milk proteins upon photo-oxidation. Journal of Agricultural and Food Chemistry, (55), 10968-10976.

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