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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Simonsen, M., Mailund, T. & Pedersen, C. S. (2008). Rapid Neighbour-Joining. In Proceedings of the 8th International Workshop on Algorithms in Bioinformatics (WABI) (pp. 113-122)

Greunke, K., Braren, I., Alpers, I., Blank, S., Sodenkamp, J., Bredehorst, R. & Spillner, E. (2008). Recombinant IgY for improvement of immunoglobulin-based analytical applications. Clinical Biochemistry, 41(14-15), 1237-44. https://doi.org/10.1016/j.clinbiochem.2008.07.004

Bramsen, J. B., Kjems, J., Ørntoft, T. F., Andersen, L. D. & Ostenfeld, M. S. (2008). Reintroduction of bladder cancer-downregulated miR-145 results in massive cell death of bladder cancer cell lines. APMIS, 417-417. https://doi.org/10.1111/j.1600-0463.2008.001165_13.x

Kragh, H., Kjærgaard, P. C., Nielsen, H. & Nielsen, K. H. (2008). Science in Denmark: A Thousand-Year History. Aarhus Universitetsforlag.

Nielsen, K. H., Kjaer, C. R. & Dahlgaard, J. (2008). Scientists' understanding of public communication of science and technology. Abstract from PCST-10 Bridges to the future, Malmø, Sweden.

Andersen, E. S., Nielsen, M. M., Dong, M., Besenbacher, F., Kjems, J. & Gothelf, K. V. (2008). Software package for the design and analysis of DNA origami structures. Paper presented at FNANO 2008, Snowbird, United States.

Otero Martin, R., Xu, W., Lukas, M., Kelly, R. E. A., Laegsgaard, E., Stensgaard, I., Kjems, J., Kantorovich, L. N. & Besenbacher, F. (2008). Specificity of Watson-Crick Base Pairing on a Solid Surface Studied at the Atomic Scale. Angewandte Chemie, 47(50), 9673-9676. https://doi.org/10.1002/anie.200803333

Christensen, P. A., Pedersen, J. S., Christiansen, G. & Otzen, D. (2008). Spectroscopic Evidence for the Existence of an Obligate Pre-Fibrillar Oligonomer during Glucagon Fibrillation. FEBS Letters, (582), 1341-1345.

Andersen, L. N. & Mandjes, M. (2008). Structural properties of reflected Lévy processes. (pp. 1-22). Thiele Centre, Institut for Matematiske Fag, Aarhus Universitet.

Odadzic, D., Bramsen, J. B., Smicius, R., Bus, C., Kjems, J. & Engels, J. W. (2008). Synthesis of 2'-O-modified adenosine building blocks and application for RNA interference. Bioorganic & Medicinal Chemistry, 16(1), 518-529. https://doi.org/10.1016/j.bmc.2007.09.019

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