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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Sand, A., Holt, M. K., Johansen, J., Fagerberg, R., Pedersen, C. N. S., Brodal, G. S. & Mailund, T. (2013). Algorithms for Computing the Triplet and Quartet Distances for Binary and General Trees. Biology, 2(4), 1189-1209. https://doi.org/10.3390/biology2041189

Tataru, P., Sand, A., Hobolth, A., Mailund, T. & Pedersen, C. N. S. (2013). Algorithms for Hidden Markov Models Restricted to Occurrences of Regular Expressions. Biology, 2(4), 1282-1295. https://doi.org/10.3390/biology2041282

Fang, X., Wang, J., O'Carroll, I. P., Mitchell, M., Zuo, X., Wang, Y., Yu, P., Liu, Y., Rausch, J. W., Dyba, M. A., Kjems, J., Schwieters, C. D., Seifert, S., Winans, R. E., Watts, N. R., Stahl, S. J., Wingfield, P. T., Byrd, R. A., Le Grice, S. F. J. ... Wang, Y.-X. (2013). An unusual topological structure of the HIV-1 Rev response element. Cell, 155(3), 594-605. https://doi.org/10.1016/j.cell.2013.10.008

Morgan, S. R., Dooley, E. P., Hocking, P. M., Inglehearn, C. F., Ali, M., Sorensen, T. L.-M., Meek, K. M. & Boote, C. (2013). An x-ray scattering study into the structural basis of corneal refractive function in an avian model. Biophysical Journal, 104(12), 2586-94. https://doi.org/10.1016/j.bpj.2013.04.053

Neuhäuser, D., Hirsch, C., Gloaguen, C. & Schmidt, V. (2013). A parametric copula approach for modelling shortest-path trees in telecommunication networks. In International Conference on Analytical and Stochastic Modeling Techniques and Applications (pp. 324-336)

Sand, A., Pedersen, C. N. S., Mailund, T., Brodal, G. S. & Fagerberg, R. (2013). A practical O(n log² n) time algorithm for computing the triplet distance on binary trees. BMC Bioinformatics, 14(Suppl 2), S18. https://doi.org/10.1186/1471-2105-14-S2-S18

Karlsen, K. K., Okholm, A. H., Kjems, J. & Wengel, J. (2013). A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid). Bioorganic & Medicinal Chemistry, 21(20), 6186-6190. http://www.sciencedirect.com/science/article/pii/S096808961300388X#MMCvFirst

Zou, L., Luo, Y., Chen, M., Wang, G., Ding, M., Petersen, C. C., Kang, R., Dagnaes-Hansen, F., Zeng, Y., Lv, N., Ma, Q., Le, D. Q. S., Besenbacher, F., Bolund, L., Jensen, T. G., Kjems, J., Pu, W. T. & Bünger, C. (2013). A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds. Scientific Reports, 3, Article 2243. https://doi.org/10.1038/srep02243

Zhang, Y., Panneerselvam, K., Ogaki, R., Rigau, L. H., van der westen, R., Jensen, B. E. B., Mian Teo, B., Zhu, M. & Stadler, B. M. (2013). Assembly of Poly(dopamine)/Poly(N-isopropyl acrylamide) Mixed Films and their Temperature Dependent Interaction with Proteins, Liposomes and Cells. Langmuir, 29(32), 10213-10222. https://doi.org/10.1021/la402118u

Xu, W., Tan, Q., Yu, M., Sun, Q., Kong, H., Lægsgaard, E., Stensgaard, I., Kjems, J., Wang, J., Wang, C. & Besenbacher, F. (2013). Atomic-scale structures and interactions between the guanine quartet and potassium. Chemical Communications, 49(65), 7210-7212. https://doi.org/10.1039/c3cc43302a

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Kalinka Hansen