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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Nguyen, V. H., Oh, S., Mahato, M., Tabassian, R., Yoo, H., Lee, S. G., Garai, M., Kim, K. J. & Oh, I. K. (2024). Functionally antagonistic polyelectrolyte for electro-ionic soft actuator. Nature Communications, 15(1), Article 435. https://doi.org/10.1038/s41467-024-44719-z

Dilamian, M., Montazer, M., Yousefi, H., Otzen, D. E. & Morshedi, D. (2024). Functional porous protein nanofibrils/polysaccharides aerogel beads for efficient dyes removal from water. Materials Advances, 5(18), 7199-7221. https://doi.org/10.1039/d4ma00380b

Zhang, X., Li, C., Yang, B., Wang, W., Zhao, J., Zhao, W., Dong, M. & Han, X. (2024). Functional Prototissues Using Artificial Cells as Building Blocks and Their Biomedical Applications. Advanced Functional Materials, 34(40), Article 2405823. https://doi.org/10.1002/adfm.202405823

Nielsen, K. H. (2024). Fupkonferencer. Weekendavisen, Sekion 4 (Ideer), 5.

Nowak, J. S. & Otzen, D. E. (2024). Helping proteins come in from the cold: 5 burning questions about cold-active enzymes. BBA advances, 5, Article 100104. https://doi.org/10.1016/j.bbadva.2023.100104

Huang, Y., Xu, H., Wang, Y., Xing, Z., Fang, R., Lai, H., Qian, M., Dong, M., Carraro, M., Skrydstrup, T., Daasbjerg, K. & Xin, Z. (2024). Hierarchical Superhydrophilic/Superaerophobic Ni₃S₂/VS₂ Nanorod-Based Bifunctional Electrocatalyst Supported on Nickel Foam for Overall Urea Electrolysis. Inorganic Chemistry, 63(40), 19002-19010. https://doi.org/10.1021/acs.inorgchem.4c03400

Nielsen, K. H. (2024). Hip hurra for videnskabsformidling. Aktuel Naturvidenskab, 2023(4), 40-42.

Xin, Z., Kou, J., Xu, H., Liu, Q., Carraro, M., Dong, M., Daasbjerg, K., Skrydstrup, T. & Huang, Y. (2024). Hollow CoFe Oxide Prisms for Cross-Dehydrogenative Coupling Reactions of 1,2,3,4-Tetrahydroisoquinolines under Mild Conditions. Inorganic Chemistry, 63(39), 18489-18494. https://doi.org/10.1021/acs.inorgchem.4c03061

Nielsen, K. H. (2024). Hormoner eller ej. Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2024). Hvid dominans i jernstøberiet. Weekendavisen, Sektion 4 (Ideer), 5.

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