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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Nam, K., Munch, K., Hobolth, A., Dutheil, J. Y., Veeramah, K. R., Woerner, A. E., Hammer, M. F., Mailund, T., Schierup, M. H. & Great Ape Genome Diversity Project (2015). Extreme selective sweeps independently targeted the X chromosomes of the great apes. Proceedings of the National Academy of Sciences (PNAS), 112(20). https://doi.org/10.1073/pnas.1419306112

Koefoed, L., Kongsfelt, M., Ulstrup, S., Cabo, A. G., Cassidy, A., Whelan, P. R., Bianchi, M., Dendzik, M., Pizzocchero, F., Jørgensen, B., Bøggild, P., Hornekær, L., Hofmann, P., Pedersen, S. U. & Daasbjerg, K. (2015). Facile electrochemical transfer of large-area single crystal epitaxial graphene from Ir(111). Journal of Physics D: Applied Physics, 48(11), Article 115306. https://doi.org/10.1088/0022-3727/48/11/115306

Feng, H., Wu, C., Zhang, P., Mi, J. & Dong, M. (2015). Facile hydrothermal synthesis and formation mechanisms of Bi₂Te₃, Sb₂Te₃ and Bi₂Te₃-Sb₂Te₃ nanowires. RSC Advances, 5(121), 100309-100315. https://doi.org/10.1039/c5ra20014h

Jiang, Z., Wang, M., Cheng, H., Li, J., Aslan, H., Lv, H., Yao, Y., Shao, L., Huang, Y. & Dong, M. (2015). Facile Preparation of TiO2 Nanoclusters on Graphene Templates for Photodegradation of Organic Compounds. Journal of Materials Science & Technology, 31(8), 840-844. https://doi.org/10.1016/j.jmst.2015.01.011

Hirsch, C., Neuhäuser, D., Gloaguen, C. & Schmidt, V. (2015). First passage percolation on random geometric graphs and an application to shortest-path trees. Advances in Applied Probability, 47(2), 328-354.

Nielsen, H., Nielsen, K. H., Nielsen, K. & Jensen, H. S. (2015). Forandringens vinde: Nye teknologihistorier. Praxis - Nyt Teknisk Forlag.

Nielsen, K. H. (2015). Forandringens vinde. Videnskab.dk [online]. http://videnskab.dk/blog/forandringens-vinde

Nielsen, K. H. & Busch, J. (2015). Forskningsfriheden flyver. Aktuel Naturvidenskab, (5), 5.

Sukosd, Z., Andersen, E. S., Seemann, S. E., Jensen, M., Hansen, M. B., Gorodkin, J. & Kjems, J. (2015). Full-length RNA structure prediction of the HIV-1 genome reveals a conserved core domain. Nucleic Acids Research, 43(21), 10168-10179. https://doi.org/10.1093/nar/gkv1039

Seviour, T., Hansen, S. H., Yang, L., Yau, Y. H., Wang, V. B., Stenvang, M. R., Christiansen, G., Marsili, E., Givskov, M., Chen, Y., Otzen, D., Nielsen, P. H., Shochat, S. G., Kjelleberg, S. & Dueholm, M. S. (2015). Functional Amyloids Keep Quorum Sensing Molecules in Check. Journal of Biological Chemistry, 290, 6457-6469. https://doi.org/10.1074/jbc.M114.613810

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