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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Otzen, D., Nesgaard, L., Andersen, K. K., Hansen, J. H., Christiansen, G., Doe, H. & Sehgal, P. (2008). Aggregation of S6 in a quasi-native state by monomeric SDS. Biochimica et Biophysica Acta - Biomembranes, (1784), 400-414.

Andersen, K. K., Westh, P. & Otzen, D. (2008). A global study of myoglobin-surfactant interactions. Langmuir, 15, 399-407.

Kristensen, T. G. & Pedersen, C. S. (2008). All-to-all RF Distance Methods. Poster session presented at Bioinformatics 2008, Warszawa, Poland.

Hobolth, A. (2008). A Markov chain Monte Carlo Expectation Maximization Algorithm for Statistical Analysis of DNA Sequence Evolution with Neighbor-Dependent Substitution Rates. Journal of Computational and Graphical Statistics, 17(1), 138-162.

Pedersen, J. S. & Otzen, D. (2008). Amyloid - a state in many guises: survival of the fittest fibril fold. Protein Science, 17, 1-9.

Larsen, P., Nielsen, J. L. & Otzen, D. (2008). Amyloid-like adhesins in floc-forming and filamentous bacteria in activated sludge. Applied and Environmental Microbiology, 74, 1517-1526.

Finster, K. (2008). Anaerobic bacteria and archaea in cold ecosystems. In Psychrophiles: From Biodiversity to Biotechnology (pp. 103-119). Springer. https://doi.org/10.1007/978-3-540-74335-4_7

Nielsen, K. H. & Ebbesen, M. (2008). Analyse: Etik. Er nanoteknologi et tveægget sværd? Politiken, 8.

Dupont, D. M., Kjems, J., Andreasen, P. & Madsen, J. C. B. (2008). A new way of targeting protease-receptor binding: Selection of uPA binding RNA aptamers inhibiting its association to its receptor uPAR. APMIS, 443-443.

Haaning, S., Radutoiu, S., Hoffmann, S. V., Dittmer, J., Giehm, L., Otzen, D. & Stougaard, J. (2008). An unusual intrinsically disordered protein from the model legume Lotus japonicus stabilizes proteins in vitro. Journal of Biological Chemistry, 283(45), 31142-52. https://doi.org/10.1074/jbc.M805024200

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