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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Hirsch, C. (2015). A Harris-Kesten theorem for confetti percolation. Random Structures Algorithms, 47(2), 361-385.

Howard, K. A. (2015). Albumin: The next-generation delivery technology. Therapeutic Delivery, 6(3), 265-268. https://doi.org/10.4155/tde.14.124

Košmrlj, A., Cordsen, P., Kyrsting, A., Otzen, D., Oddershede, L. B. & Jensen, M. H. (2015). A monomer-trimer model supports intermittent glucagon fibril growth. Scientific Reports, 5, 1-6. Article 9005. https://doi.org/10.1038/srep09005

Wang, X., Weber, J. K., Liu, L., Dong, M., Zhou, R. & Li, J. (2015). A novel form of β-strand assembly observed in Aβ_33-42 adsorbed onto graphene. Nanoscale, 7(37), 15341-15348. https://doi.org/10.1039/c5nr00555h

Ebbesen, M., Sundby, A., Pedersen, F. S. & Andersen, S. (2015). A Philosophical Analysis of Informed Consent for Whole Genome Sequencing in Biobank Research by use of Beauchamp and Childress' Four Principles of Biomedical Ethics. Journal of Clinical Research and Bioethics, 6(244). https://doi.org/10.4172/2155-9627.1000244

Xu, R., Taskin, M. B., Dagnæs-Hansen, F., Meyer, T., Bach, L. W., Axelsen, S. M., Besenbacher, F. & Chen, M. (2015). Application of a Novel 3D Biofunctional Scaffold for hiPS-MSC Fibrogenesis. Abstract from 2015 4th TERMIS World Congress , Boston, United States. http://online.liebertpub.com/toc/tea/21/S1

Aller, P., Sanchez-Weatherby, J., Foadi, J., Winter, G., Lobley, C. M. C., Axford, D., Ashton, A. W., Bellini, D., Brandao-Neto, J., Culurgioni, S., Douangamath, A., Duman, R., Evans, G., Fisher, S., Flaig, R., Hall, D. R., Lukacik, P., Mazzorana, M., McAuley, K. E. ... Walsh, M. A. (2015). Application of in situ diffraction in high-throughput structure determination platforms. Methods in molecular biology (Clifton, N.J.), 1261, 233-53. https://doi.org/10.1007/978-1-4939-2230-7_13

Liu, L., Li, Y., Xia, D., Bortolini, C., Zhang, S., Yang, Y., Pedersen, J. S., Wang, C., Besenbacher, F. & Dong, M. (2015). A self-assembled nanopatch with peptide-organic multilayers and mechanical properties. Nanoscale, 7(6), 2250-2254. https://doi.org/10.1039/c4nr03797a

Hirsch, C., Neuhäuser, D., Gloaguen, C. & Schmidt, V. (2015). Asymptotic properties of Euclidean shortest-path trees in random geometric graphs. Statistics probability letters, 107, 122-130.

Sun, M., Wang, M., Chen, M., Dagnaes-Hansen, F., Le, D. Q. S., Baatrup, A., Horsman, M. R., Kjems, J. & Bünger, C. (2015). A tissue-engineered therapeutic device inhibits tumor growth in vitro and in vivo. Acta Biomaterialia, 18, 21-29. https://doi.org/10.1016/j.actbio.2015.02.004

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