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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Vogt, S., Bobbili, M. R., Stadlmayr, G., Stadlbauer, K., Kjems, J., Rüker, F., Grillari, J. & Wozniak-Knopp, G. (2021). An engineered CD81-based combinatorial library for selecting recombinant binders to cell surface proteins: Laminin binding CD81 enhances cellular uptake of extracellular vesicles. Journal of Extracellular Vesicles, 10(11), Article e12139. https://doi.org/10.1002/jev2.12139

Focht, D., Neumann, C., Lyons, J., Eguskiza Bilbao, A., Blunck, R., Malinauskaite, L., Schwarz, I. O., Javitch, J. A., Quick, M. & Nissen, P. (2021). A non-helical region in transmembrane helix 6 of hydrophobic amino acid transporter MhsT mediates substrate recognition. EMBO Journal, 40(1), Article e105164. https://doi.org/10.15252/embj.2020105164

González-García, M., Morales-Vicente, F., Pico, E. D., Garay, H., Rivera, D. G., Grieshober, M., Olari, L. R., Groß, R., Conzelmann, C., Krüger, F., Zech, F., Bozzo, C. P., Müller, J. A., Zelikin, A., Raber, H., Kubiczek, D., Rosenau, F., Münch, J., Stenger, S. ... Otero-Gonzalez, A. J. (2021). Antimicrobial activity of cyclic-monomeric and dimeric derivatives of the snail-derived peptide cm-p5 against viral and multidrug-resistant bacterial strains. Biomolecules, 11(5), Article 745. https://doi.org/10.3390/biom11050745

Yu, Z., Centola, M., Valero, J., Matthies, M., Šulc, P. & Famulok, M. (2021). A Self-Regulating DNA Rotaxane Linear Actuator Driven by Chemical Energy. Journal of the American Chemical Society, 143(33), 13292-13298. https://doi.org/10.1021/jacs.1c06226

Choong, F. X., Huzell, S., Rosenberg, M., Eckert, J. A., Nagaraj, M., Zhang, T., Melican, K., Otzen, D. E. & Richter-Dahlfors, A. (2021). A semi high-throughput method for real-time monitoring of curli producing Salmonella biofilms on air-solid interfaces. Biofilm, 3, Article 100060. https://doi.org/10.1016/j.bioflm.2021.100060

Valero, J., Civit, L., Dupont, D. M., Selnihhin, D., Reinert, L. S., Idorn, M., Israels, B. A., Bednarz, A. M., Bus, C., Asbach, B., Peterhoff, D., Pedersen, F. S., Birkedal, V., Wagner, R., Paludan, S. R. & Kjems, J. (2021). A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry. Proceedings of the National Academy of Sciences (PNAS), 118(50), Article e2112942118. https://doi.org/10.1073/pnas.2112942118

Zhang, Y., Zhang, D., Wang, Y., Liu, Q., Li, Q. & Dong, MD. (2021). Atomic-Scale Friction of Black and Violet Phosphorus Crystals: Implications for Phosphorus-Based Devices and Lubricants. ACS Applied Nano Materials, 4(9), 9932-9937. https://doi.org/10.1021/acsanm.1c02593

Nielsen, K. H. (2021). Atomic Urbanism under Greenland’s Ice Cap: Camp Century and Cold War Architectural Imagination. In R. Brook, M. Dodge & J. Hogg (Eds.), Cold War Cities: Politics, Culture and Atomic Urbanism, 1945-1965 (pp. 182-199). Routledge. https://doi.org/10.4324/9780203701478

Ahmed, S. Z., Hjorth, A., Rafner, J. F., Weidner, C. A., Kragh, G., Jensen, J. H. M., Bobroff, J., Nielsen, K. H. & Sherson, J. F. (2021). A training programme for early-stage researchers that focuses on developing personal science outreach portfolios. Science Communication https://doi.org/10.48550/arXiv.2103.03109

Otzen, D. E., Morshedi, D., Mohammad-Beigi, H. & Aliakbari, F. (2021). A Triple Role for a Bilayer: Using Nanoliposomes to Cross and Protect Cellular Membranes. Journal of Membrane Biology, 254(1), 29-39. https://doi.org/10.1007/s00232-020-00159-6

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Kalinka Hansen