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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Schiølin, K. H. (2016). Cum Deus Calculat, Fit Mundus, or The Will to Technology: Diagnosis and Cure. A Peer-Reviewed Journal About, 5(1). http://www.aprja.net/?p=3083

Bortolini, C. & Dong, M. (2016). Cystine oligomers successfully attached to peptide cysteine-rich fibrils. Frontiers of Chemical Science and Engineering, 10(1), 99-102. https://doi.org/10.1007/s11705-016-1554-6

Hall, J. P., Keane, P. M., Beer, H., Buchner, K., Winter, G., Sorensen, T. L., Cardin, D. J., Brazier, J. A. & Cardin, C. J. (2016). Delta chirality ruthenium 'light-switch' complexes can bind in the minor groove of DNA with five different binding modes. Nucleic Acids Research, 44(19), 9472-9482. https://doi.org/10.1093/nar/gkw753

Kim, J.-Y., Sahu, S., Yau, Y.-H., Wang, X., Shochat, S. G., Nielsen, P. H., Dueholm, M. S., Otzen, D. E., Lee, J., Delos Santos, M. M. S., Yam, J. K. H., Kang, N.-Y., Park, S.-J., Kwon, H., Seviour, T. W., Yang, L., Givskov, M. & Chang, Y.-T. (2016). Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11. Journal of the American Chemical Society, 138(1), 402-407. https://doi.org/10.1021/jacs.5b11357

Jordal, P. L., Dyrlund, T. F., Winge, K., Larsen, M. R., Danielsen, E. H., Wells, J. A., Otzen, D. E. & Enghild, J. J. (2016). Detection of proteolytic signatures for Parkinson's disease. Future Neurology, 11(1), 15-32. https://doi.org/10.2217/fnl.16.3

Wang, J., Zhu, Z., Bortolini, C., Hoffmann, S. V., Amari, A., Zhang, H. X., Liu, L. & Dong, M. D. (2016). Dimensionality of carbon nanomaterial impacting on the modulation of amyloid peptide assembly. Nanotechnology, 27(30), Article 304001. https://doi.org/10.1088/0957-4484/27/30/304001

Nielsen, K. H. (2016). Diskurs. Weekendavisen, Ideer, 10.

Nielsen, K. H. (2016). Dna. Weekendavisen, Ideer, 10.

Okholm, A. H. & Kjems, J. (2016). DNA nanovehicles and the biological barriers. Advanced Drug Delivery Reviews, 106(Part A), 183-191. Article 16. https://doi.org/10.1016/j.addr.2016.05.024

Kodal, A. L. B., Rosen, C. B., Mortensen, M. R., Tørring, T. & Gothelf, K. V. (2016). DNA-Templated Introduction of an Aldehyde Handle in Proteins. ChemBioChem, 17(14), 1338-1342. https://doi.org/10.1002/cbic.201600254

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