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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Li, Y., Liu, L., Subramani, R., Pan, Y., Liu, B., Yang, Y., Wang, C., Mamdouh, W., Besenbacher, F. & Dong, M. (2011). Building layer-by-layer 3D supramolecular nanostructures at the terephthalic acid/stearic acid interface. Chemical Communications, 47(32), 9155-9157. https://doi.org/10.1039/c1cc11443c

Yao, Y., Chen, D., Zhang, S., Li, Y., Tu, P., Liu, B. & Dong, M. (2011). Building the first hydration shell of deprotonated glycine by the MCMM and ab initio methods. Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical, 115(19), 6213-21. https://doi.org/10.1021/jp1117097

Nygaard, J. V., Uyar, T., Chen, M., Cloetens, P., Kingshott, P. & Besenbacher, F. (2011). Characterisation of internal morphologies in electrospun fibers by X-ray tomographic microscopy. Nanoscale, 3. https://doi.org/10.1039/c1nr10304k

Bramsen, J. B. & Kjems, J. (2011). Chemical modification of small interfering RNA. Methods in Molecular Biology, 721(1), 77-103. https://doi.org/10.1007/978-1-61779-037-9_5

Nielsen, K. H. (2011). City under the Ice: The Closed World of Camp Century. Abstract from HSS Annual Meeting, Cleveland, OH, United States. http://www.hssonline.org/Meeting/2011HSSMeeting/Program%20Abstracts.pdf

Locke, D. P., Hillier, L. W., Warren, W. C., Yang, S.-P., Wang, Z., Chinwalla, A. T., Minx, P., Mitreva, M., Cook, L., Delehaunty, K. D., Fronick, C., Schmidt, H., Fulton, L. A., Fulton, R. S., Nelson, J. O., Magrini, V., Pohl, C., Graves, T. A., Markovic, C. ... de Jong, P. J. (2011). Comparative and demographic analysis of orang-utan genomes. Nature, 469, 529-533. https://doi.org/10.1038/nature09687

Tataru, P. C. & Hobolth, A. (2011). Comparison of methods for calculating conditional expectations of sufficient statistics for continuous time Markov chains. BMC Bioinformatics, 12(1), 465. https://doi.org/10.1186/1471-2105-12-465

Jepsen, M. DE., Beerens, N., Besenbacher, F., Kjems, J. & Birkedal, V. (2011). Conformational changes of HIV-1 RNA upon tRNA primer annealing studied by ensemble and single-molecule FRET.

Tørring, T. (2011). Controlling Function and Structure with DNA.

Bald, I., Wang, Y.-G., Dong, M., Rosen, C. B., Ravnsbaek, J. B., Zhuang, G.-L., Gothelf, K. V., Wang, J.-G. & Besenbacher, F. (2011). Control of Self-Assembled 2D Nanostructures by Methylation of Guanine. Small, 7(7), 939-49. https://doi.org/10.1002/smll.201002033

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Kalinka Hansen