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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Nørnberg, P., Vendelboe, A. L., Gunnlaugsson, H. P., Merrison, J. P., Finster, K. & Jensen, S. K. (2011). Possible formation of hematite and maghemite from goethite/ferrihydrite under temperate conditions. Abstract from Euroclay 2011, Antalya, Turkey.

Le, D. Q. S., Andersen, M. Ø., Baatrup, A., Chen, M., Chen, M., Lysdahl, H., Besenbacher, F., Bünger, C. & Kjems, J. (2011). Process for Modifying the Surface Morphology of a Medical Device: PA 2011 70752.

Otzen, D. (2011). Protein-surfactant interactions a tale of many states. BBA General Subjects, 1814(5), 562-91. https://doi.org/10.1016/j.bbapap.2011.03.003

Braren, I., Greunke, K., Grunwald, T., Pilette, C., Mempel, M., Bredehorst, R., Ring, J., Spillner, E. & Ollert, M. (2011). Quantitation of serum IgE by using chimeras of human IgE receptor and avian immunoglobulin domains. Analytical Biochemistry, 412(2), 134-140. https://doi.org/10.1016/j.ab.2010.12.013

Barndorff-Nielsen, O. E. & Basse-O'Connor, A. (2011). Quasi Ornstein-Uhlenbeck processes. Bernoulli, 17(3), 916-941. https://doi.org/10.3150/10-BEJ311

Simonsen, M. & Pedersen, C. N. S. (2011). Rapid Computation of Distance Estimators from Nucleotide and Amino Acid Alignments. In Proceedings of the 26th Annual ACM Symposium on Applied Computing Association for Computing Machinery.

Becker, A. L., Orlotti, N. I., Folini, M., Cavalieri, F., Zelikin, A. N., Johnston, A. P. R. J., Zaffaroni, N. & Caruso, F. (2011). Redox-Active Polymer Microcapsules for the Delivery of a Survivin-Specific siRNA in Prostate Cancer Cells. ACS Nano, 5, 1335-1344.

Jakob, T., Blank, S., Spillner, E. & Ollert, M. (2011). Rekombinante Insektengift-Allergene: Ist das Bienen- oder doch das Wespengift schuld? Der Deutsche Dermatologe, 59, 759-761.

Hofmann, S. C., Pfender, N., Weckesser, S., Jakob, T., Blank, S., Spillner, E. & Huss-Marp, J. (2011). Reply. Journal of Allergy and Clinical Immunology, 128(1), 248. https://doi.org/10.1016/j.jaci.2011.02.020

Dupont, D. M., Madsen, J. B., Bøtkjær, K. A., Tavitian, B., Duconge, F., Kjems, J. & Andreasen, P. (2011). RNA aptamers to the cancer-associated proteins UPA and PAI-1 with therapeutic potential. Nucleic Acid Therapeutics, 21(5).

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