Publications

Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

Sort by: Date | Author | Title

Mazo-Vargas, A., Langmüller, A. M., Wilder, A., van der Burg, K. R. L., Lewis, J. J., Messer, P. W., Zhang, L., Martin, A. & Reed, R. D. (2022). Deep cis-regulatory homology of the butterfly wing pattern ground plan. Science, 378(6617), 304-308. https://doi.org/10.1126/science.abi9407

Nielsen, K. H. (2022). Den forsvundne Indiana Jones. Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2022). Den norske grødstrid. Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2022). Det værst tænkelige. Weekendavisen, Sektion 4 (Ideer), 5.

Fjelstrup, S., Dupont, D. M., Bus, C., Enghild, J. J., Jensen, J. B., Birkenkamp-Demtröder, K., Dyrskjøt, L. & Kjems, J. (2022). Differential RNA aptamer affinity profiling on plasma as a potential diagnostic tool for bladder cancer. NAR Cancer, 4(3), Article zcac025. https://doi.org/10.1093/narcan/zcac025

Harms, M., Hansson, R. F., Carmali, S., Almeida-Hernández, Y., Sanchez-Garcia, E., Münch, J. & Zelikin, A. N. (2022). Dimerization of the Peptide CXCR4-Antagonist on Macromolecular and Supramolecular Protraction Arms Affords Increased Potency and Enhanced Plasma Stability. Bioconjugate Chemistry, 33(4), 594-607. https://doi.org/10.1021/acs.bioconjchem.2c00034

Pérez-García, F., Brito, L. F., Irla, M., Jorge, J. M. P. & Sgobba, E. (2022). Editorial: Promising and sustainable microbial feedstocks for biotechnological processes. Frontiers in Microbiology, 13, Article 973723. https://doi.org/10.3389/fmicb.2022.973723

Irla, M. & Wendisch, V. F. (2022). Efficient cell factories for the production of N-methylated amino acids and for methanol-based amino acid production. Microbial Biotechnology, 15(8), 2145-2159. https://doi.org/10.1111/1751-7915.14067

Grønnemose, A. L., Østerlund, E. C., Otzen, D. E. & Jørgensen, T. J. D. (2022). EGCG has Dual and Opposing Effects on the N-terminal Region of Self-associating α-synuclein Oligomers. Journal of Molecular Biology, 434(23), Article 167855. https://doi.org/10.1016/j.jmb.2022.167855

Wu, R., Ou, X., Zhang, L., Song, X., Wang, Z., Dong, M. & Liu, L. (2022). Electric Field Effect on Inhibiting the Co-fibrillation of Amyloid Peptides by Modulating the Aggregation Pathway. Langmuir, 38(40), 12346-12355. https://doi.org/10.1021/acs.langmuir.2c02055

Displaying results 631 to 640 out of 3503