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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Kronqvist, N., Otikovs, M., Chmyrov, V., Chen, G., Andersson, M., Nordling, K., Landreh, M., Sarr, M., Jörnvall, H., Wennmalm, S., Widengren, J., Meng, Q., Rising, A., Otzen, D., Knight, S. D., Jaudzems, K. & Johansson, J. (2014). Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation. Nature Communications, 5, Article 3254. https://doi.org/10.1038/ncomms4254

Tørring, T., Helmig, S. W., Ogilby, P. R. & Gothelf, K. V. (2014). Singlet Oxygen in DNA Nanotechnology. Accounts of Chemical Research, 47(6), 1799-1806. https://doi.org/10.1021/ar500034y

Miclaus, T., Bochenkov, V., Ogaki, R., Howard, K. & Sutherland, D. S. (2014). Spatial Mapping and Quantification of Soft and Hard Protein Coronas at Silver Nanocubes. Nano Letters, 14(4), 2086-2093. https://doi.org/10.1021/nl500277c

Nawroth, I., Mueller, F., Basyuk, E., Beerens, N., Rahbek, U. L., Darzacq, X., Bertrand, E., Kjems, J. & Schmidt, U. (2014). Stable assembly of HIV-1 export complexes occurs cotranscriptionally. RNA, 20(1), 1-8. https://doi.org/10.1261/rna.038182.113

Basse-O'Connor, A., Graversen, S.-E. & Pedersen, J. (2014). Stochastic integration on the real line. Theory of Probability and Its Applications, 58(2), 193-213. https://doi.org/10.1137/S0040585X97986540

Nam, K., Munch, K., Hobolth, A., Y. Dutheil, J., Veeramah, K., Woerner, A., F. Hammer, M., Ape Genome Diversity Project, G., Mailund, T. & H. Schierup, M. (2014). Strong selective sweeps associated with ampliconic regions in great ape X chromosomes. ArXiv.

Lyons, J. A., Parker, J. L., Solcan, N., Brinth, A., Li, D., Shah, S. T., Caffrey, M. & Newstead, S. (2014). Structural basis for polyspecificity in the POT family of proton-coupled oligopeptide transporters. EMBO Reports, 15(8), 886-893. https://doi.org/10.15252/embr.201338403

Ling, M., Santl-Temkiv, T., Finster, K. & Boesen, T. (2014). Structural studies of bacterial ice nucleation proteins and their relevance for atmospheric processes. Poster session presented at 2nd Ice Binding Conference, Sapporo, Japan.

Zhang, S., Jiang, Z., Besenbacher, F. & Dong, M. (2014). Study Sub-Membrane Structure and Corresponding Functions of Conductive Bacteria Cable by SPMs. Biophysical Journal, 106(2), 799A-799A.

Mian Teo, B., Lynge, M. E., Rigau, L. H. & Stadler, B. M. (2014). Subcompartmentalized Surface-Adhering Polymer Thin Films Toward Drug Delivery Applications. In Layer-by-Layer Films for Biomedical Applications Wiley.

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