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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Zelikin, A. N. & Städler, B. (2020). Artificial Biology: Molecular Design and Cell Mimicry. Small, 16(27), Article 2003442. https://doi.org/10.1002/smll.202003442

Venø, M. T., Reschke, C. R., Morris, G., Connolly, N. M. C., Su, J., Yan, Y., Engel, T., Jimenez-Mateos, E. M., Harder, L. M., Pultz, D., Haunsberger, S. J., Pal, A., Heller, J. P., Campbell, A., Langa, E., Brennan, G. P., Conboy, K., Richardson, A., Norwood, B. A. ... Henshall, D. C. (2020). A systems approach delivers a functional microRNA catalog and expanded targets for seizure suppression in temporal lobe epilepsy. Proceedings of the National Academy of Sciences (PNAS), 117(27), 15977-15988. https://doi.org/10.1073/pnas.1919313117

Jia, Y., Xiong, X., Wang, D., Duan, X., Sun, K., Li, Y., Zheng, L., Lin, W., Dong, M., Zhang, G., Liu, W. & Sun, X. (2020). Atomically Dispersed Fe-N₄ Modified with Precisely Located S for Highly Efficient Oxygen Reduction. Nano-Micro Letters, 12(1), Article 116. https://doi.org/10.1007/s40820-020-00456-8

Hobolth, A., Guo, Q., Kousholt, A. & Jensen, J. L. (2020). A Unifying Framework and Comparison of Algorithms for Non-negative Matrix Factorisation. International Statistical Review, 88(1), 29-53. https://doi.org/10.1111/insr.12331

Nielsen, B. S., Møller, T. & Kjems, J. (2020). Automated One-Double-Z Pair BaseScope™ for CircRNA In Situ Hybridization. In B. Schnack Nielsen & J. Jones (Eds.), In Situ Hybridization Protocols (pp. 379-388). Humana Press, Inc.. https://doi.org/10.1007/978-1-0716-0623-0_24

Ramsay, E., Raviña, M., Sarkhel, S., Hehir, S., Cameron, N. R., Ilmarinen, T., Skottman, H., Kjems, J., Urtti, A., Ruponen, M. & Subrizi, A. (2020). Avoiding the pitfalls of siRNA delivery to the retinal pigment epithelium with physiologically relevant cell models. Pharmaceutics, 12(7), Article 667. https://doi.org/10.3390/pharmaceutics12070667

Nielsen, N. S., Poulsen, E. T., Lukassen, M. V., Chao Shern, C., Mogensen, E. H., Weberskov, C. E., DeDionisio, L., Schauser, L., Moore, T. C. B., Otzen, D. E., Hjortdal, J. & Enghild, J. J. (2020). Biochemical mechanisms of aggregation in TGFBI-linked corneal dystrophies. Progress in Retinal and Eye Research, 77, Article 100843. https://doi.org/10.1016/j.preteyeres.2020.100843

Tian, X., Chen, X., Feng, Y., Duan, Y., Dong, M., Pan, G. & Liu, L. (2020). Biomimetic fabrication of dynamic biointerfaces with optional and diversified bioactivities through reversible covalent and bioorthogonal chemistry. Chemical Engineering Journal, 398, Article 125620. https://doi.org/10.1016/j.cej.2020.125620

Yoshimoto, R., Rahimi, K., Hansen, T. B., Kjems, J. & Mayeda, A. (2020). Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-wide Interspersed Repeats. iScience, 23(7), Article 101345. https://doi.org/10.1016/j.isci.2020.101345

Just, J., Yan, Y., Farup, J., Sieljacks, P., Sloth, M., Venø, M., Gu, T., de Paoli, F. V., Nyengaard, J. R., Bæk, R., Jørgensen, M. M., Kjems, J., Vissing, K. & Drasbek, K. R. (2020). Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles. Scientific Reports, 10(1), Article 5835. https://doi.org/10.1038/s41598-020-62456-3

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Kalinka Hansen