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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Langmüller, A. M., Champer, J., Lapinska, S., Xie, L., Metzloff, M., Champer, S. E., Liu, J., Xu, Y., Du, J., Clark, A. G. & Messer, P. W. (2022). Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations. eLife, 11, Article e71809. https://doi.org/10.7554/eLife.71809

Sønderby, T. V., Rasmussen, H. Ø., Frank, S. A., Skov Pedersen, J. & Otzen, D. E. (2022). Folding steps in the fibrillation of functional amyloid: denaturant sensitivity reveals common features in nucleation and elongation. Journal of Molecular Biology, 434(2), Article 167337. https://doi.org/10.1016/j.jmb.2021.167337

Nielsen, K. H. (2022). For hårdt, for blødt, for stift. Weekendavisen, Sektion 4 (Ideer), 10.

Zhang, D., Li, Z., Klausen, L. H., Li, Q. & Dong, M. (2022). Friction behaviors of two-dimensional materials at the nanoscale. Materials Today Physics, 27, Article 100771. https://doi.org/10.1016/j.mtphys.2022.100771

Blevins, A. S., Bassett, D. S., Scott, E. K. & Vanwalleghem, G. C. (2022). From calcium imaging to graph topology. Network Neuroscience, 6(4), 1125-1147. https://doi.org/10.1162/netn_a_00262

Otzen, D. E. (2022). Functional amyloid in a lipid-like environment: a merry dance of many steps. Essays in Biochemistry, 66(7), 1035-1046. https://doi.org/10.1042/EBC20220062

Sønderby, T. V., Najarzadeh, Z. & Otzen, D. E. (2022). Functional Bacterial Amyloids: Understanding Fibrillation, Regulating Biofilm Fibril Formation and Organizing Surface Assemblies. Molecules, 27(13), Article 4080. https://doi.org/10.3390/molecules27134080

Krebs, J. & Hirsch, C. (2022). Functional central limit theorems for persistent Betti numbers on cylindrical networks. Scandinavian Journal of Statistics, 49(1), 427-454. https://doi.org/10.1111/sjos.12524

Märcher, A., Kumar, V., Andersen, V. L., El-Chami, K., Nguyen, T. J. D., Skaanning, M. K., Rudnik-Jansen, I., Nielsen, J. S., Howard, K. A., Kjems, J. & Gothelf, K. V. (2022). Functionalized Acyclic (l)-Threoninol Nucleic Acid Four-Way Junction with High Stability In Vitro and In Vivo. Angewandte Chemie - International Edition, 61(24), Article 202115275. https://doi.org/10.1002/anie.202115275

Nielsen, K. H. (2022). Gaia overvinder alt. Weekendavisen, Sektion 4 (Ideer), 5.

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Kalinka Hansen