Find

AUNAB - Aarhus University Network on Artificial Biology

Publications

Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

Sort by: Date | Author | Title

Vu Quang, H., Vinding, M. S., Xia, D., Nielsen, T., Ullisch, M. G., Dong, M., Nielsen, N. C. & Kjems, J. (2016). Chitosan-coated Poly(lactic-co-glycolic acid) Perfluorooctyl Bromide Nanoparticles for Cell Labeling in 19F Magnetic Resonance Imaging. Carbohydrate Polymers, 136, 936-944. https://doi.org/10.1016/j.carbpol.2015.09.076

Louw, A. M., Kolar, M. K., Novikova, L. N., Kingham, P. J., Wiberg, M., Kjems, J. & Novikov, L. N. (2016). Chitosan polyplex mediated delivery of miRNA-124 reduces activation of microglial cells in vitro and in rat models of spinal cord injury. Nanomedicine: Nanotechnology, Biology and Medicine, 12(3), 643-653. https://doi.org/10.1016/j.nano.2015.10.011

Ebbesen, K. K., Kjems, J. & Hansen, T. B. (2016). Circular RNAs: Identification, biogenesis and function. BBA General Subjects, 1859(1), 163-168. https://doi.org/10.1016/j.bbagrm.2015.07.007

Søndergaard, D. A., Knudsen, M. & Pedersen, C. S. (2016). Computational Classification of P-Type ATPases. Methods in Molecular Biology, 1377, 493-502. https://doi.org/10.1007/978-1-4939-3179-8

Knudsen, M., Søndergaard, D. A., Tofting-Olesen, C., Hansen, F. T., Brodersen, D. E. & Pedersen, C. S. (2016). Computational discovery of specificity-conferring sites in non-ribosomal peptide synthetases. Bioinformatics, 32(3), 325-329. https://doi.org/10.1093/bioinformatics/btv600

Andersen, K. K., Vad, B., Omer, S. & Otzen, D. E. (2016). Concatemers of Outer Membrane Protein A Take Detours in the Folding Landscape. Biochemistry, 55(51), 7123–7140. https://doi.org/10.1021/acs.biochem.6b01153

Yang, X., Li, Q., Hu, G., Wang, Z., Yang, Z., Liu, X., Dong, M. & Pan, C. (2016). Controlled synthesis of high-quality crystals of monolayer MoS2 for nanoelectronic device application. Science china-Materials, 59(3), 182-190. https://doi.org/10.1007/s40843-016-0130-1

Song, X., Wang, Z., Liu, X., Dong, M. & Wang, L. (2016). Controlling the intracell diffusion behaviour of Ag monomers on Si(111)-(7 x 7) by voltage pulse manipulation. Applied Physics Letters, 109(10), 177-180. Article 103105. https://doi.org/10.1063/1.4962497

Westberg, M., Bregnhøj, M., Blazquez-Castro, A., Breitenbach, T., Etzerodt, M. & Ogilby, P. R. (2016). Control of Singlet Oxygen Production in Experiments Performed on Single Mammalian Cells. Journal of Photochemistry and Photobiology, A: Chemistry, 321, 297-308. https://doi.org/10.1016/j.jphotochem.2016.01.028

Nielsen, K. H. (2016). CRISPR. Weekendavisen, Ideer, 10.

Displaying results 1701 to 1710 out of 3467

171

Revised 03.03.2026

-

Kalinka Hansen