Publications

Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

Sort by: Date | Author | Title

Tian, P., Lindorff-Larsen, K., Boomsma, W., Jensen, M. H. & Otzen, D. E. (2016). A Monte Carlo Study of the Early Steps of Functional Amyloid Formation. PLoS One, 11(1), Article e0146096. https://doi.org/10.1371/journal.pone.0146096

Bienk, K., Hvam, M. L., Pakula, M. M., Dagnæs-Hansen, F., Wengel, J., Malle, B. M., Kragh-Hansen, U., Cameron, J., Bukrinski, J. T. & Howard, K. (2016). An albumin-mediated cholesterol design-based strategy for tuning siRNA pharmacokinetics and gene silencing. Journal of Controlled Release, 232, 143-151. https://doi.org/10.1016/j.jconrel.2016.04.013

Nielsen, K. H. (2016). Antropocæn. Weekendavisen, Ideer, 10.

Hansen, T. B., Venø, M. T., Jensen, T. I., Schaefer, A., Damgaard, C. K. & Kjems, J. (2016). Argonaute-associated short introns are a novel class of gene regulators. Nature Communications, 7, Article 11538. https://doi.org/10.1038/ncomms11538

Cheng, W., Xu, R., Li, D., Bortolini, C., He, J., Dong, MD., Besenbacher, F., Huang, Y. & Chen, M. (2016). Artificial extracellular matrix delivers TGFb1 regulating myofibroblast differentiation. RSC Advances, 6(26), 21922-21928. https://doi.org/10.1039/c5ra26164c

Neuhäuser, D., Hirsch, C., Gloaguen, C. & Schmidt, V. (2016). A stochastic model for multi-hierarchical networks. Methodology and Computing in Applied Probability, 18(4), 1129-1151.

Kristoffersen, E. L. (2016). A Study of Designed DNA Nanostructures and their use in Enzyme Activity Detection.

Nielsen, K. H. & Kjærgaard, A. L. (2016). At se og fejle som en velfærdsstat: Moderniseringsbestræbelser og -problemer i Grønland efter anden verdenskrig. TEMP. Tidsskrift for historie, (12), 41-58.

Nielsen, K. H. (2016). Bevidsthed. Weekendavisen, Ideer, 10.

Ebbesen, M. (2016). Bioethics across Cultures – Philosophical and Empirical Justification of the Four Principles of Biomedical Ethics. Journal of Clinical Research and Bioethics, 7(1), 259-261. https://doi.org/10.4172/2155-9627.1000259

Displaying results 1681 to 1690 out of 3467

169