Opportunities

Project Opportunities in Ditlev E. Brodersen laboratory.

The non-ribosomal peptide synthetases (NRPSs) constitute a large group of biosynthetic enzymes found in both bacteria and fungi responsible for producing a wide range of bioactive polypeptides. Well-known examples of products synthesized by NRPSs are the antibiotic penicillin and the immunosuppressant cyclosporin, used during kidney transplant surgery to prevent organ rejection. However, the vast majority of fungal NRPSs are currently unexplored and this group of enzymes therefore holds a huge potential for development of novel pharmaceuticals. In NANORIPES we are investigating the structure and function of NRPS enzymes present in species of the crop pathogen, Fusarium with the aim of becoming better at predicting the biomedical potential of unknown NRPSs.

The project involves a wide range of methods in biochemistry and structural biology. The overall aim is structural and functional studies of individual specificity domains (A-domains) from NRPS enzymes. The experimental approach involves cloning, protein expression, and purification with the aim of crystallization and finally structure determination by x-ray crystallography. Structures of ligand-bound enzyme domains will then subsequently be studied in collaboration with chemists identifying the bound amino acids.

Interested undergraduate and graduate students are always welcome to contact us for a chat about the options in the laboratory.

Contact information:

Associate professor

Ditlev Egeskov Brodersen

 

Department of Molecular Biology and Genetics - structural biology

Gustav Wieds Vej 10

Building 3130, room 205

8000 Aarhus C

Denmark

deb@mb.au.dk

Postdoc

Frederik Teilfeldt Hansen

 

Department of Molecular Biology and Genetics - structural biology

Gustav Wieds Vej 10

Building 3131, room 2.2.06

8000 Aarhus C

Denmark

fth@mb.au.dk