September 2014-September 2015
Malthe Tvind Hvas
The combined effect of nitrite and hypercapnia on methaemoglobin formation and nitrite uptake in the striped catfish, Pangasianodon hypophthalmus
An investigation of the combined effects of hypercapnia and Nitrite on the Striped catfish (Pangasianodon hypophthalmus). In striped catfish, elevated CO2 causes a respiratory acidosis where blood pH is recovered within 24-48 hours by accumulation of HCO3-, which is counterbalanced by a decrease in Cl-. Elevated nitrite levels can occasionally occur in aquaculture systems, entering fish by competing with Cl- for anion uptake at the same branchial Cl-/HCO3- exchanger utilized in nitrite uptake. In the blood, nitrite oxidises the haem iron forming methaemoglobin, which cannot bind oxygen. Since striped catfish need to retain HCO3- to protect pH during hypercapnia, we hypothesized that elevated CO2 would protect against nitrite toxicity through a decreased activity of the Cl-/HCO3- exchanger. This was investigated in fish cannulated in the dorsal aorta allowing for repeated blood sampling for plasma ion and metheamoglobin measurements during combined and isolated exposures to 0.9 mM nitrite and 3% hypercapnia.
Louise Damgaard Thomasen
The respiratory cardiovascular system of the air-breathing fish Pangasianodon hypophthalmus
Air-breathing in fish is thought to have evolved in response to aquatic hypoxia many times, resulting in a variety of air-breathing organs (ABO) and equally diverse circulatory bauplans. These are variously argued to enhance the cardiac oxygen supply, prevent the loss of oxygen over the gills and to prevent the mixing of deoxygenated and oxygenated blood. I will investigate the bauplan of the facultative air-breather Pangasianodon hypophthalmus and the autonomic regulation of the cardiovascular system. This will be done with erosion casting, high contrast CT-scanning and dissection and the use of small vessel myography.
Mads Kuhlmann Andersen
Does elevated temperature compromise aerobic performance in the facultative air-breathing fish, Pangasianodon hypophthalmus?
During the next century, aquatic temperatures are predicted to increase by as much as 5°C, consequently increasing the oxygen demand of fish whilst decreasing oxygen availability. This dual obstacle has led several authors to propose that the absolute aerobic scope (AAS), and by extension whole organism fitness, will be reduced in future supraoptimal temperatures. Tropical species are further predicted to be particularly vulnerable to increasing temperatures as they unavoidably live closer to their upper critical thermal limit than temperate species. In the present study, standard and maximum metabolic rates (SMR and MMR, respectively) of the striped catfish (Pangasianodon hypophthalmus), a tropical bimodal breather, will be estimated using intermittent closed respirometry combined with a chase protocol, and using two-phase swim respirometry using a critical swim speed (Ucrit) protocol.
No. | Student name | Thesis title | Thesis contents | Supervisor(s) | Status |
1 | Ho Thanh Hoa | Effect of salinity on digestive enzyme activities of stripped catfish (Pangasianodon hypophthalmus) fingerlings | - Chymotrypsine, pepsine and amylase activities in stomach and intestine of tra catfish juveniles (8-10 g) at different salinities. | A/Prof.Dr. Do Thi Thanh Huong | Completed 12/07/2014 |
2 | Le Hoang Quy | Study on the reproduction of swamp eel (Monopterus albus Zuiew, 1973) | - Effects of maturation feeding on the ovary development of swamp eel. | A/Prof.Dr. Do Thi Thanh Huong | Completed 12/07/2014 |
3 | Duong Tuan Thinh | Effects of salinity on physiological parameters of stripped catfish (Pangasianodon hypophthalmus) in pond conditions. | - Changes of hematological parameters and glucose. concentrations in striped catfish blood under freshwater and brackish water environment. | A/Prof.Dr. Do Thi Thanh Huong | Completed 13/07/2014 |
4 | Nguyen Thanh Binh | Effects of feeding rate on digestive enzyme activities and growth of snakehead (Channa striata) | - Effects of feeding rate on digestive enzyme activities of snakehead. | Prof.Dr. Nguyen Thanh Phuong
| Completed 13/07/2014 |
5 | Đoan Van Chang | Effects of salinity on osmoregulation and growth of pond loach (Misgurnus anguillicaudatus) | - Salinity tolerance of pond loach. | A/Prof.Dr. Do Thi Thanh Huong | Completed 22/11/2014 |
6 | Vo Truong Chinh | Effect of temperature on digestive enzyme activities and growth of snakehead (Channa striata). | - Digestive enzyme (pepsin, chymotrypsine and α-amylase) activities and feed digestibility of snakehead at different temperatures. | A/Prof.Dr. Do Thi Thanh Huong | Completed 23/11/2014 |
7 | Đang Van Quoi | Effects of aeration supplied on water quality parameters and growth performance of striped catfish (Pangasianodon hypophthalmus) in grow-out pond. | - Effect of aeration supplied on grow-out pond dissolved oxygen and water quality parameters. | Dr. Vo Nam Son A/Prof.Dr. Do Thi Thanh Huong | Completed 13/07/2014 |
8 | Tran Thi Kieu Linh | Effects of temperature and salinity on the physiological parameters and growth of striped catfish (Pangasianodon hypophthalmus) fingerlings | - Changes of hematological parameters changed in striped catfish (Pangasianodon hypophthalmus) fingerlings under different combined temperature and salinity conditions. | Prof.Dr. Nguyen Thanh Phuong
| Completed
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9 | Nguyen Thi Bi | Toxic gases (CO2 and H2S) in striped catfish (Pangasianodon hypophthalmus) pond and their effects on fish physiology. | - Variation of CO2 and H2S in striped catfish grow-out ponds. | Prof.Dr. Nguyen Thanh Phuong Dr. Vo Nam Son | Completed
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10 | Vo Quoc Hao | Effect of temperature on haematological parameters and growth of giant gourami (Osphronemus goramy) fingerlings | - Temperature tolerance of giant gourami fingerlings. | A/Prof.Dr. Do Thi Thanh Huong | Completed
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11 | Duong Thi My Tien | Effects of β-glucan on hematological parameters and stress tolerance of striped catfish (Pangasianodon hypophthalmus) fingerlings. | - Effects of different β-glucan concentrations on hematological parameters of striped catfish fingerlings. | A/Prof.Dr. Do Thi Thanh Huong | Completed
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12 | Le Thi Kieu Trang | Effects of combined nitrite and temperature on hematological parameters and growth of striped catfish (Pangasianodon hypophthalmus) fingerlings. | - LC50-96h of the combined nitrite and temperature on striped catfish fingerlings. | Prof.Dr. Nguyen Thanh Phuong
| Completed
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13 | Tran Thi Kim Tuyen | Effect of salinity on hematology, osmolality, and growth of basa catfish (Pangasius bocourti) fingerlings. | - Salinity tolerance of basa catfish fingerlings. | A/Prof.Dr. Do Thi Thanh Huong | Completed
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14 | Nguyen Thi Thuy Vu | Effect of nitrite on hematological parameters and growth of Chitala Chitala fingerlings
| - LC50-96h of the nitrite on Chitala chitala fingerlings. | A/Prof.Dr. Do Thi Thanh Huong | Completed
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15 | Nguyen The Dien | Application of recirculating system in super intensive nursing of striped catfish (Pagasianodon hypophthalmus) from larvae to fingerling stage. | - Nursing striped catfish fry at different densities to fingerling stage in recirculating system. | Prof.Dr. Nguyen Thanh Phuong
| On-going |
16 | Nguyen Thi Xuan Bieu | Effects of hypercapnia on hematological parameters and growth of basa catfish (Pangasius bocourti) | - Effects of hypercapnia on hematological parameters of basa catfish. | A/Prof.Dr. Do Thi Thanh Huong | On-going |
17 | Nguyen Tam Em | Chemicals use and withdrawal times of flofenicol, doxycycline and amoxicillin in red tilapia (Oreochromis niloticus × Oreochromis mossambicus) cage farming | - Chemicals use in red tilapia cage farming. | A/Prf.Dr. Do Thi Thanh Huong | On-going |
18 | Đinh Thi Truc Giang | The adaptability of giant gourami (Osphronemus goramy) to elevated temperature and salinity | - Salinity and/or elevated temperature tolerance of giant gourami fingerlings. | Prof.Dr. Nguyen Thanh Phuong
| On-going |
19 | Nguyen Tran Phuong Thao | Effects of nitrite on hematological parameters and growth of basa catfish (Pangasius bocourti) | - LC50-96h of nitrite on basa catfish fish at 96h exposure. | A/Prof.Dr. Do Thi Thanh Huong | On-going |
